Table 1.
Target | Primer name | Primer sequence and label | References |
---|---|---|---|
HVT (HVT070 gene) |
HVT-F3 | 5′-ATAAATTATATCGCTAGGACAGAC-3′ | (38) |
HVT-B3 | 5′-ACGATGTGCTGTCGTCTA-3′ | ||
HVT-FIP | 5′-6-FAM-CCAGGGTATGCATATTCCATAACA GTTTTCCAAACGACCTTTATCCCA-3′ |
||
HVT-BIP | 5′-Biosg-CCAGAAATTGCACGCACGAGTTTT AGAATTTGTGCATTTAGCCTT-3′ |
||
HVT-LF | 5′-TTGAGAAGAGGATCTGACTG-3′ | ||
HVT-LB | 5′-GCGTCATTGGTTTTACATTT-3′ | ||
Vaxxitek® (HVT065 gene and intergenic region + cloning vector) | Vaxxitek-F3 | 5′-CCGAACAAACTTCATCGCTA-3′ | This study |
Vaxxitek-B3 | 5′-GCTATTGCTTTATTTGTAACCAT-3′ | ||
Vaxxitek-FIP | 5′-6-FAM-CCCAAAGACCTCTATGAACATTTATTTTTGCAAAGAGATGCGTGTG-3′ | ||
Vaxxitek-BIP | 5′-Biosg-TGTCGACTCTAGAGGATCCGAAAATTTTGTTAACAACAACAATTGCATTCA-3′ | ||
Vaxxitek-LF | 5′-TACTCAACGGCGCGTGTA-3′ | ||
Vaxxitek-LB | 5′-CACACCTCCCCCTGAACCTG-3′ |