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. 2022 Jun 23;9:873163. doi: 10.3389/fvets.2022.873163

Table 1.

LAMP primer sets used in this study.

Target Primer name Primer sequence and label References
HVT
(HVT070 gene)
HVT-F3 5′-ATAAATTATATCGCTAGGACAGAC-3′ (38)
HVT-B3 5′-ACGATGTGCTGTCGTCTA-3′
HVT-FIP 5′-6-FAM-CCAGGGTATGCATATTCCATAACA
GTTTTCCAAACGACCTTTATCCCA-3′
HVT-BIP 5′-Biosg-CCAGAAATTGCACGCACGAGTTTT
AGAATTTGTGCATTTAGCCTT-3′
HVT-LF 5′-TTGAGAAGAGGATCTGACTG-3′
HVT-LB 5′-GCGTCATTGGTTTTACATTT-3′
Vaxxitek® (HVT065 gene and intergenic region + cloning vector) Vaxxitek-F3 5′-CCGAACAAACTTCATCGCTA-3′ This study
Vaxxitek-B3 5′-GCTATTGCTTTATTTGTAACCAT-3′
Vaxxitek-FIP 5′-6-FAM-CCCAAAGACCTCTATGAACATTTATTTTTGCAAAGAGATGCGTGTG-3′
Vaxxitek-BIP 5′-Biosg-TGTCGACTCTAGAGGATCCGAAAATTTTGTTAACAACAACAATTGCATTCA-3′
Vaxxitek-LF 5′-TACTCAACGGCGCGTGTA-3′
Vaxxitek-LB 5′-CACACCTCCCCCTGAACCTG-3′