Table 2.
Primer sequences used for quantitative PCR assays.
| Encoding gene | Primer sequence (5′-3′) |
Accession No./
Reference |
|---|---|---|
| Digestive enzymes | ||
| AMY2A | F: CGGAGTGGATGTTAACGACTGG R: ATGTTCGCAGACCCAGTCATTG |
NM_001001473.2 |
| PNLIP | F: GCATCTGGGAAG↓GAACTAGGG R: TGAACCACAAGCATAGCCCA |
NM_001277382.1 |
| CCK | F: AGGTTCCACTGGGAGGTTCT R: CGCCTGCTGTTCTTTAGGAG |
XM_015281332.1 |
| CELA1 | F: AGCGTAAGGAAATGGGGTGG R: GTGGAGACCCCATGCAAGTC |
XM_015300368.1 |
| Barrier functions | ||
| Cathelicidins-2 | F: AGGAGAATGGGGTCATCAGG R: GGATCTTTCTCAGGAAGCGG |
NM_001024830.3 |
| β-defensin-1 | F: AAACCATTGTCAGCCCTGTG R: TTCCTAGAGCCTGGGAGGAT |
NM_204993.1 |
| Occludin | F: ACGGCAAAGCCAACATCTAC R:ATCCGCCACGTTCTTCAC |
XM_031604121.1 |
| CLDN-1 | F: GGTGAAGAAGATGCGGATGG R: TCTGGTGTTAACGGGTGTGA |
NM_001013611 |
| MUC-2 | F: AAACAACGGCCATGTTTCAT R: GTGTGACACTGGTGTGCTGA |
NM_001318434 |
| JAM-2 | F: AGACAGGAACAGGCAGTGCT R: TCCAATCCCATTTGAGGCTA |
XM_031556661.1 |
| FABP-2 | F: AGGCTCTTGGAACCTGGAAG R: CTTGGCTTCAACTCCTTCGT |
NM_001007923 |
| Cytokines | ||
| IL-6 | F: AGGACGAGATGTGCAAGAAGTTC R: TTGGGCAGGTTGAGGTTGTT |
NM_204628.1 |
| IL-8 | F: CTGGCCCTCCTCCTGGTT R: GCAGCTCATTCCCCATCTTTAC |
XM_015281283.2 |
| IL-10 | F: GCTGAGGGTGAAGTTTGAGG R: AGACTGGCAGCCAAAGGTC |
XM_025143715.1 |
| IL-1β | F:GCTCTACATGTCGTGTGTGATGAG R: 50-TGTCGATGTCCCGCATGA |
NM_204524 |
| TNF-α | F: CGTTTGGGAGTGGGCTTTAA R: GCTGATGGCAGAGGCAGAA |
NM_204267.1 |
| House keeping | ||
| GAPDH | F: CAACCCCCAATGTCTCTGTT R: TCAGCAGCAGCCTTCACTAC |
NM205518 |
| TBP | F: GTCCACGGTGAATCTTGGTT R: GCGCAGTAGTACGTGGTTCTC |
Acc:8484 |
| E. coli | ||
| 16S rRNA | F: GACCTCGGTTTAGTTCACAGA R: CACACGCTGACGCTGACCA |
(39) |
| Total bacteria | ||
| 16S rRNA | F: CGGYCCAGACTCCTACGGG R: TTACCGCGGCTGCTGGCAC |
(40) |
| Enterobacteriaceae | ||
| 16S rRNA | F: CATTGACGTTACCCGCAGAAGAAGC R: CTCTACGAGACTCAAGCT TGC |
|
| Bacteroides species | ||
| 16S rRNA | F: GAGAGGAAGGTCCCCCAC R: CGCTACTTGGCTGGTTCAG |
|
| Lactobacillus species | ||
| 16S rRNA | F: CACCGCTACACATGGAG R: AGCAGTAGGGAATCTTCCA |
|
| E. coli O78 virulence | ||
| papC | F: GACGGCTGTACTGCAGGGTGTGGCG R: ATATCCTTTCTGCAGGGATGCAATA |
(41) |
| iroN | F: AATCCGGCAAAGAGACGAACCGCCT R: GTTCGGGCAACCCCTGCTTTGACTTT |
|
| iutA | F: GGCTGGACATCATGGGAACTGG R: CGTCGGGAACGGGTAGAATCG |
|
| iss | F: CAGCAACCCGAACCACTTGATG R: AGCATTGCCAGAGCGGCAGAA |
AMY2A, alpha 2A amylase; PNLIP, pancreatic lipase; CCK, cholecystokinin; CELA1, chymotrypsin-like elastase family, member 1; CLDN-1, claudins-1; MUC-2, mucin-2; JAM-2, junctional adhesion molecule-2; FABP-2, fatty acid binding protein-2; IL, interleukin; TNF-α, tumor necrosis factor alpha; GAPDH, glyceraldehyde-3- phosphate dehydrogenase; TBP, TATA-binding protein.