Skip to main content
. 2022 May 23;20(4):307–318. doi: 10.18502/ijrm.v20i4.10903

Figure 6.

Figure 6

Lectin histochemistry revealed the distribution patterns of the reaction with Con A, PNA and WGA in head of the sperms. Con A reacted with the mannose residues at posterior acrosomal membrane in the acrosomal reacted-sperms (yellow arrow). Red arrow shows the acrosomal intact sperms. PNA stained with acrosomal content and the acrosomal intact sperms react with PNA at the anterior pole of the nucleous where the acrosome located (yellow arrow). The sperms without PNA reactivity are spontaneously acrosomal reacted (red arrow). The non-capacitate sperms also react with WGA (yellow arrow in the bottom pictures). Scale bar is 10 µm, PNA: peanut agglutinin; WGA: wheat germ agglutinin.