Table 3.

Hormone analysis results for the different 25(OH)D groups (n = 119)

[2.2in,lr]Variables 25(OH)D (ng/ml)	< 10 (n = 28)	10-30 (n = 65)	> 30 (n = 26)	P-value	Post hoc tests
LH (ng/ml)	8.18 $\pm$ 3.57 ${}^i$	8.67 $\pm$ 3.75 ${}^d$	5.36 $\pm$ 2.62 ${}^j$	$<$ 0.001 ${}^a$	i & j (p $<$ 0.001) d & j (p $<$ 0.001)
FSH (ng/ml)	5.71 $\pm$ 2.82	7.03 $\pm$ 3.43	5.98 $\pm$ 2.84	0.22 ${}^b$	-
SHBG (nmol/ml)	10.53 $\pm$ 6.61 ${}^c$	11.51 $\pm$ 5.77 ${}^k$	22.09 $\pm$ 6.24 ${}^e$	$<$ 0.001 ${}^a$	c & e (p $<$ 0.001) k & e (p $<$ 0.001)
Tt (ng/ml)	2.58 $\pm$ 1.08 ${}^c$	3.30 $\pm$ 0.92 ${}^d$	5.87 $\pm$ 2.08 ${}^e$	$<$ 0.001 ${}^b$	c & d & e (p $<$ 0.001)
E2 (pg/ml)	30.17 $\pm$ 13.27 ${}^c$	29.26 $\pm$ 14.28 ${}^g$	21.58 $\pm$ 8.70 ${}^f$	0.01 ${}^b$	c & f (p = 0.01) g & f (p $<$ 0.001)
Tt/E2 ratio	0.09 $\pm$ 0.04 ${}^c$	0.13 $\pm$ 0.06 ${}^d$	0.30 $\pm$ 0.14 ${}^e$	$<$ 0.001 ${}^A$	c & d (p $<$ 0.001) c & e (p $<$ 0.001) d & e (p $<$ 0.001)
Tt/LH ratio	0.41 $\pm$ 0.35 ${}^c$	0.52 $\pm$ 0.43 ${}^h$	1.57 $\pm$ 1.43 ${}^e$	$<$ 0.001 ${}^b$	c & e (p $<$ 0.001) h & e (p $<$ 0.001)
FAI% (Tt/SHBG. 100)	34.84 $\pm$ 25.08	36.77 $\pm$ 22.46	29.03 $\pm$ 13.80	0.41 ${}^b$	-
Data presented as Mean $\pm$ SD. 25(OH)D: 25-hydroxy vitamin D, LH: Luteinizing hormone, FSH: Follicle-stimulating hormone, SHBG: Sex hormone-binding globulin, Tt: Testosterone, E2: Estradiol, FAI: Free androgen index. Differences between the 3 groups were tested using one-way analysis of variance (ANOVA) (a), Welch and Brown-Forsythe (A), Kruskal-Wallis test (b). Post hoc tests for normal distribution data (a): Least Significant Difference test (LSD), (A): Dunnett T3. Pairwise comparison for non-normal distribution data were tested using Mann-Whitney test (b). P $<$ 0.05 was considered statistically significant