TABLE 5.
Effects of flumazenil (FMZ) and thiosemicarbazide (TSC) on the anticonvulsive action of 6d against MES and PTZ-induced seizures in mice.
| Compound | Dose (mg/kg) | Test time (h) | MES model | PTZ model |
|---|---|---|---|---|
| Saline + 6d | 30 | 0.5 | 5/5 c | 5/5 |
| FMZ + 6d a | 30 | 0.5 | 0/5*** | 0/5*** |
| Saline + 6d | 30 | 0.5 | 5/5 | 5/5 |
| TSC + 6d b | 30 | 0.5 | 0/5*** | 0/5*** |
The number of animals tested per group was five. Significance was determined by Fisher’s exact test. ***p < 0.001 vs. group of 6d (pretreatment with saline).
a
Mice was pretreated with flumazenil (10 mg/kg, single administration) 15 min before seizure induction.
b
Mice was pretreated with TSC (25 mg/kg/day for 3 days).
c
Number of animals protected/number of animals tested.