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. 2022 Jun 23;13:848915. doi: 10.3389/fphar.2022.848915

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Copyright © 2022 Xu, Zhang, Wang, Chen, Xu, Sun, Yang, Lin, Wang and Huang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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HG-induced EMT in NRK-52E cells and GPS inhibited HG-induced EMT in NRK-52E cells starting at 12.5 μM. (A,B) Protein levels of E-cad, vimentin, α-SMA, and FN under the HG conditions at various times were detected by Western blot. **p < 0.01 and *p < 0.05 vs. 0 h. (C) Migration ability of NRK-52E cells under the HG conditions at various times was detected by a cell scratch test. **p < 0.01 and *p < 0.05 vs. 0 h. (D) Protein levels of α-SMA and vimentin after GPS intervention were detected by Western blot. **p < 0.01 vs. Ctrl, ^## p < 0.01 vs. HG. Data were expressed as mean ± SD.