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. 2022 Jun 23;13:848915. doi: 10.3389/fphar.2022.848915

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Copyright © 2022 Xu, Zhang, Wang, Chen, Xu, Sun, Yang, Lin, Wang and Huang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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GPS inhibited HG-induced activation of the CK2/NF-κB pathway in NRK-52E cells. (A) Effects of GPS on p65 distribution in the nucleus and cytoplasm were detected by Western blot. **p < 0.01 vs. Ctrl. ^## p < 0.01 and ^# p < 0.05 vs. HG. (B) Effects of GPS on p65 distribution in the nucleus and cytoplasm were detected by immunofluorescence. Scale bar: 20 μm. (C) Effect of GPS on the NF-κB p65 DNA binding activity was detected by EMSA. (D) Effect of GPS on the NF-κB p65 transcriptional activity was detected by luciferase reporter assay. **p < 0.01 vs. Ctrl. ^## p < 0.01 and ^# p < 0.05 vs. HG. (E,F) Protein levels of IκBα, p-IκBα, and p-CK2α after GPS intervention were detected by Western blot. **p < 0.01 and *p < 0.05 vs. Ctrl. ^## p < 0.01 and ^# p < 0.05 vs. HG. **p < 0.01 vs. Ctrl. ^## p < 0.01 and ^# p < 0.05 vs. HG. Data were expressed as mean ± SD.