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. 2022 Jun 23;13:936806. doi: 10.3389/fgene.2022.936806

TABLE 1.

Summary of samples from five consecutive generations of the Troutlodge May spawning population used in this study.

Generation Study Comment
2013 WGS Selected parents for the 2015 generation were used for WGS (Individuals and pools; N = 20)
2015 WGS, SNP validation 1) Selected parents (Individuals and pools; N = 20) and families (pools; N = 480) were used for WGS; 2) Validate SNPs associated with BCWD resistance using 60 families (10 offspring per family; N = 600) with intermediate BCWD survival rates
2017 SNP validation Validate SNPs associated with BCWD resistance using 60 families (10 offspring per family; N = 600) with intermediate BCWD survival rates
2019 SNP validation Validate SNPs associated with BCWD resistance using 9 families (92 offspring per family; N = 828) with intermediate BCWD survival rates
2021 MAS Evaluation of MAS using Pooled BCWD challenge (50 families; 20 fish per family; N = 1,000)