Figure 4.

Inhibition of HEV replication by Zinc compounds. (A) qRT-PCR detection of the HEV sense and antisense strand RNA levels in ORF4-Huh7 cells infected with a g1-HEV clinical isolate and treated for 24 h with indicated concentration of ZnO(NP), ZnO(TP), and ZnSO_4. HEV sense/antisense RNA values were normalized to that of the GAPDH and represented as mean ± SEM of three independent experiments. (B) Measurement of Gaussia Luciferase (G-Luc) activity in Huh7 cells expressing in vitro-synthesized capped RNA of a g3-HEV-Luc replicon and treated for 24 h with indicated concentration of ZnO(NP), ZnO(TP), and ZnSO₄. G-Luc values were normalized to that of the cell viability and represented as mean ± SEM of three independent experiments. Mock-treated cells were used as a control.