Fig. 6.

RqPCR confirmed repression of OSR2 across the carcinogenic pathway (a) and the hypermethylation of its associated CpG site was confirmed using pyrosequencing (c). A sample pyrogram from an OSR2 methylation assay in normal FT sample (b). Sequence analysed is along the bottom of figure. The blue bars represent the target CpG dinucelotides and the figures above these represent the percentage methylation at that position. A figure highlighted in red indicates a result that needs review and may constitute a failed reading. In the initial validation within the “study cohort” all values were included, and a mean methylation calculated for each sample. However, within the larger validation sample set combinations of the methylation position scores were assessed to confirm which was most discriminatory. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)