Table 1.
Validation parameters for the methodology used to quantify HMF and FF.
| Analyte | Linear range (mmol L−1) | Equation of the line | R2 | LOD (mmol L−1) |
LOQ (mmol L−1) |
Level (mmol L−1) |
Rep. RSD(%) n = 10 | Int. prec. RSD(%) n=10 | Rec. RSD(%) n = 3 |
|---|---|---|---|---|---|---|---|---|---|
| HMF | 3.00 × 10−2 2.10 × 10−1 | y = (x-0.5562)/ 50.2139 |
0.9972 | 0.002371 | 0.035249 | 0.048 | 0.83 | 2.89 | 98.76 |
| 0.120 | 0.84 | 3.97 | 98.63 | ||||||
| 0.192 | 3.90 | 3.34 | 97.26 | ||||||
| FF | 5.00 × 10−4 3.00 × 10−2 | y = (x-0.0011)/ 50.5597 | 0.9998 | 0.000855 | 0.012651 | 0.0035 | 0.72 | 0.41 | 97.80 |
| 0.0153 | 1.23 | 1.63 | 94.56 | ||||||
| 0.0271 | 4.46 | 5.87 | 91.12 |
R2 = coefficient of determination; LOD = limit of detection; LOQ = limit of quantification; RSD = relative standard deviation; Rep. = repeatability; Int. prec. = intermediate precision; Rec. = recovery.