Subject Area:	Chemistry
More specific subject area:	Food and biomass analysis
Method name:	HPLC-DAD-based method for HMF and furfural using FDCA as internal standard
Name and reference of original method:	J.K. de Andrade, E. Komatsu, H. Perreault, Y.R. Torres, M.R. da Rosa, M.L. Felsner, In house validation from direct determination of 5-hydroxymethyl-2-furfural (HMF) in Brazilian corn and cane syrups samples by HPLC-UV. Food Chem. 190 (2016) 481–486. https://doi.org/10.1016/j.foodchem.2015.05.131
Resource availability:	Reagents: - Type I ultrapure water with resistivity of 18.2 MΩ.cm (PURELAB Option Q system) - Trisodium citrate buffer solution (Sigma-Aldrich, MW 294.10 g mol−1, purity ≥99.0%) - Glacial acetic acid (Dinâmica, UV/HPLC grade, purity of 99.5%) - HMF (Sigma-Aldrich, MW 126.11 g mol−1, purity ≥99.0%) - Furfural (Sigma-Aldrich, MW 96.08 g mol−1, purity of 99.0%) - FDCA (Sigma-Aldrich, MW 156.09 g mol−1, purity of 97.0%) Materials: - pH meter (mPA210, MS Tecnopon)