Table 1.
Oligonucleotide primers used to amplify putative virulence determinants (Experiment A: E. faecalis infection, pathology, and host cytokine gene expression in chicken embryos).
| Virulence gene | Primer(5-3) | Product size (bp) | Annealing temp (°C) |
|---|---|---|---|
| Ragg-F | AAGAAAAAGAAGTAGACCAAC | 1553 | 55 |
| Ragg-R | AAACGGCAAGACAAGTAAATA | ||
| RgelE-F | ACCCCGTATCATTGGTTT | 419 | 55 |
| RgelE-R | ACGCATTGCTTTTCCATC | ||
| RcylM-F | CTGATGGAAAGAAGATAGTAT | 742 | 53 |
| RcylM-R | TGAGTTGGTCTGATTACATTT | ||
| RcylB-F | ATTCCTACCTATGTTCTGTTA | 843 | 53 |
| RcylB-R | AATAAACTCTTCTTTTCCAAC | ||
| RcylA-F | TGGATGATAGTGATAGGAAGT | 517 | 53 |
| RcylA-R | TCTACAGTAAATCTTTCGTCA | ||
| RefaAfs-F | GACAGACCCTCACGAATA | 705 | 53 |
| RefaAfs-R | AGTTCATCATGCTGTAGTA | ||
| Rcob-F | AACATTCAGCAAACAAAGC | 1405 | 53 |
| Rcob-R | TTGTCATAAAGAGTGGTCAT | ||
| Rccf-F | GGGAATTGAGTAGTGAAGAAG | 543 | 53 |
| Rccf-R | AGCCGCTAAAATCGGTAAAAT |