Table 2.

Primers and probes for host cytokine gene expression detection by RT-qPCR (Experiment A: E. faecalis infection, pathology, and host cytokine gene expression in chicken embryos and Experiment C: E. faecalis and E. coli coinfection in chicken embryos and subsequent mortality and host cytokine gene expression in embryos and neonatal chickens).

Primers/probe	Sequence
IL-1 Forward IL-1 Reverse IL-1 Probe	5-GCTCTACATGTCGTGTGTGATGAG-3 5-TGTCGATGTCCCGCATGA-3 5-(FAM)-CCACACTGCAGCTGGAGGAAGCC-(ZEN/IBFQ)-3
IL-8 Forward IL-8 Reverse IL-8 Probe	5-GCCCTCCTCCTGGTTTCAG-3 5-TGGCACCGCAGCTCATT-3 5-(FAM)-TCTTTACCAGCGTCCTACCTTGCGACA-(ZEN/IBFQ)-3
MIP-1 Forward MIP-1 Reverse MIP-1 Probe	5-GGCAGACTACTACGAGACCAACAG-3 5-ACGGCCCTTCCTGGTGAT-3 5-(FAM)-ACACAACACCAGCATGAGGGCACTG-(ZEN/IBFQ)-3
CXCR-4 Forward CXCR-4 Reverse CXCR-4 Probe	5-TGCTGCCTCAATCCAATTCTT-3 5-CAAGGCATTTTGTGCTGATGTT-3 5-(FAM)-ACGCCTTCCTGGGTGCCAAGTTC-(ZEN/IBFQ)-3