Table 3.

Isolation percentages of E. faecalis and/or E. coli from chicken embryos in enrichment culture.

Group-time point	Shell membrane		Amnion		Yolk
	E. faecalis (%)	E. coli (%)	E. faecalis (%)	E. coli (%)	E. faecalis (%)	E. coli (%)
E. faecalis 109 CFU/mlL– 48 h	0		0		0
E. faecalis 109 CFU/mL – 6 d	40		40		80
E. faecalis 109 CFU/mL – 8 d	20				80
E.coli103 CFU/mL – 48 h		0		0		0
E.coli103 CFU/mL – 6 d		0		0		0
E.coli103 CFU/mL – 8 d		0				20
E.coli106 CFU/mL – 48 h		0		20		20
E.coli106 CFU/mL – 6 d		40		20		20
E.coli106 CFU/mL – 8 d		80				100
E.coli109 CFU/mL – 48 h		0		20		20
E.coli109 CFU/mL – 6 d		20		0		20
E.coli109 CFU/mL – 8 d		40				60
E. faecalis + E.coli (109/103) – 48 h	40	0	40	0	40	0
E. faecalis + E.coli (109/103) – 6 d	60	0	80	0	60	0
E. faecalis + E.coli (109/103) – 8 d	60	20			60	0
E. faecalis + E.coli (109/ 106) – 48hrs	20	0	20	20	20	20
E. faecalis + E.coli (109/106) – 6 d	0	0	0	0	40	20
E. faecalis + E.coli (109/106) – 8 d	0	20			60	80
E. faecalis + E.coli (109/109) – 48 h	20	20	20	20	20	20
E. faecalis + E.coli (109/109) – 6d	20	20	20	40	80	60
E. faecalis + E.coli (109/109) – 8 d	40	40			20	20

Enrichment cultures were obtained from shell membrane, amnion and yolk sac at different time points following exposure to E. faecalis and/or E. coli. The empty boxes indicate no isolation was attempted. (Experiment B: E. faecalis and E. coli coinfection in chicken embryos and subsequent mortality and pathology in neonatal).