Skip to main content
. 2022 Jun 23;15:927149. doi: 10.3389/fnmol.2022.927149

FIGURE 2.

FIGURE 2

Endogenous BDNF contributes to synaptic vesicle exocytosis. Rat hippocampal neuron cultures (16DIV) transfected with vGlut-pH and mCherry were perfused with TrkB-Fc (1 μg/ml) prior to tetanic stimulation (lightning bolt; A) or mBDNF with no stimulation (D) and imaged as indicated. Quantification of electrically-evoked synaptic vesicle exocytosis shows that pretreatment with the BDNF binding protein TrkB-Fc (1 μg/ml) for 5 min prior to tetanic stimulation reduced exocytosis (B) (*p < 0.05 by Student’s paired t-test). Representative images of rat hippocampal neurons (16DIV) transfected with mCherry (C, left) and vGlut-pH showing single boutons before (C, middle) and after (C, right) addition of exogenous mBDNF (75–100 ng/ml) with defined ROIs used for analysis (white circles). Synaptic vesicle exocytosis was increased by mBDNF without stimulation (E) (****p < 0.0001 by Student’s paired t-test). Data are mean±SD; n = 5–7 neurons per experimental group and 250–350 boutons. Scale bar = 5 μm.