Table 2. Primer and probe sequences used in quantitative RT-PCR.
Targeted gene | Sequence (5’ to 3’) | Product size (bp) | |
---|---|---|---|
FOXP3 | CACCTGGAAGAACGCCATCC | Forward | 94 |
CTCATCCACGGTCCACACAG | Reverse | ||
CCCTTCTCGCTCTCCACCCGCACA | Probe | ||
TCACCTGCTCCACGGCCTTGCTCT | Probe | ||
IL-17A | GGAATCTCCACCGCAATGAGG | Forward | 130 |
TGCTGGATGGGGACAGAGTTC | Reverse | ||
TGGTAGTCCACGTTCCCATCAGCG | Probe | ||
TGF-β | CCAGAAATACAGCAACAATTCCTG | Forward | 136 |
GCCCTCAATTTCCCCTCCAC | Reverse | ||
CTCAACCACTGCCGCACAACTCCG | Probe | ||
18s rRNA | GTTGATTAAAGTCCCTGCCCTTTG | Forward | 152 |
TCCGAGGGCCTCACTAAACC | Reverse | ||
ACACACCGCCCGTCGCTACACCG | Probe |
RT-PCR: Reverse transcription polymerase chain reaction; FOXP3: Forkhead box P3, IL-10: Interleukin 10; TGF-β: Transforming growth factor-beta