Table 2. Primer and probe sequences used in quantitative RT-PCR.
| Targeted gene | Sequence (5’ to 3’) | Product size (bp) | |
|---|---|---|---|
| FOXP3 | CACCTGGAAGAACGCCATCC | Forward | 94 |
| CTCATCCACGGTCCACACAG | Reverse | ||
| CCCTTCTCGCTCTCCACCCGCACA | Probe | ||
| TCACCTGCTCCACGGCCTTGCTCT | Probe | ||
| IL-17A | GGAATCTCCACCGCAATGAGG | Forward | 130 |
| TGCTGGATGGGGACAGAGTTC | Reverse | ||
| TGGTAGTCCACGTTCCCATCAGCG | Probe | ||
| TGF-β | CCAGAAATACAGCAACAATTCCTG | Forward | 136 |
| GCCCTCAATTTCCCCTCCAC | Reverse | ||
| CTCAACCACTGCCGCACAACTCCG | Probe | ||
| 18s rRNA | GTTGATTAAAGTCCCTGCCCTTTG | Forward | 152 |
| TCCGAGGGCCTCACTAAACC | Reverse | ||
| ACACACCGCCCGTCGCTACACCG | Probe | ||
RT-PCR: Reverse transcription polymerase chain reaction; FOXP3: Forkhead box P3, IL-10: Interleukin 10; TGF-β: Transforming growth factor-beta