REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
2-7, 2-15, 2-30, 2-36, 2-38, 2-43, 4-20	Liu et al, 2020	N/A
47D11	Wang et al., 2020	N/A
ADG-2	Rappazzo et al., 2021	N/A
B-38	Wu et al., 2020	N/A
Bamlanivimab	Eli Lilly; Primary paper: Jones et al., 2021	N/A
BD23, BD-236, BD-368-2	Cao et al., 2020	N/A
C002, C022, C101, C102, C104, C105, C123, C125, C128, C135, C140, C144, C155, C165, C210	Robbiani et al., 2020	N/A
CC6.31, CC6.33, CC12.4	Rogers et al., 2020	N/A
CnC2t1p1_B4, CnC2t1p1_D6, CnC2t1p1_E8, CnC2t1p1_E12, CnC2t1p1_G6, FnC1t2p1_D4, FnC1t2p1_G5, HbnC2t1p2_D9, HbnC3t1p1_C6, HbnC3t1p1_G4, HbnC3t1p2_B10, HbnC3t1p2_C6, MnC1t3p1_G9, MnC2t1p1_A3, MnC2t1p1_C5, MnC2t2p1_C11, MnC4t2p1_B3, MnC4t2p1_D10, MnC4t2p1_E6, MnC4t2p2_A4, MnC5t2p1_G1	Kreer et al., 2020	N/A
COV2-2050, COV2-2064, COV2-2068, COV2-2098, COV2-2130, COV2-2196, COV2-2268, COV2-2308, COV2-2354, COV2-2381, COV2-2479, COV2-2499, COV2-2531, COV2-2539, COV2-2562, COV2-2677, COV2-2678, COV2-2752, COV2-2841, COV2-2919, COV2-2952, COV2-2955	Zost et al., 2020a; Zost et al., 2020b	N/A
COVA2-29	Brouwer et al., 2020	N/A
CV-X2-106, CV07-262, CV07-270, CV38-139, CV38-142	Kreye et al., 2020	N/A
CT-P59 (Regdanvimab)	Kim et al., 2021	N/A
DH1042, DH1128, DH1138, DH1184, DH1210	Li et al., 2021	N/A
DZIF-10c	Boehringer Ingelheim; Primary paper: Kreer et al., 2020	N/A
Etesevimab	Eli Lilly; Primary paper: Shi et al., 2020	N/A
GW01	Wang et al., 2022	N/A
Casirivimab, Imdevimab	Regeneron; Primary paper: Hansen et al., 2020	N/A
LY-CoV1404 (Bebtelovimab)	Westendorf et al., 2022	N/A
MAD0004J08	Andreano et al., 2021	N/A
P2B-2F6, P2C-1F11	Ju et al., 2020	N/A
R40-1A1, R40-1A8, R40-1B4, R40-1B9, R40-1C8, R40-1D3, R40-1E1, R40-1E4, R40-1G6, R40-1G8, R40-1G12, R40-1H4, R121-1F1, R121-3F7, R121-3F11, R121-3G2, R200-1B8, R200-1B9, R200-1F9, R200-1G11, R200-4F4, R207-1C1, R207-1C4, R207-1G1, R207-2A6, R207-2A10, R207-2C2, R207-2F11, R207-2G4, R207-2H1, R259-1B9, R339-1B11, R339-3B5, R339-3C6, R410-1A8, R568-1A9, R568-1B3, R568-1C6, R568-1E8, R568-1G9, R568-2A1, R568-2A3, R568-2B9, R568-2B11, R568-2E1, R568-2E7, R568-2F1, R568-2G5, R568-2G11, R616-1A11, R616-1D6, R616-1F10, R616-1G4, R849-1C11, R849-1G7, R849-1H1R849-3H2	Vanshylla et al., 2022	N/A
REGN10954, REGN10955, REGN10964, REGN10970, REGN10971, REGN10977 REGN10986, REGN10989	Hansen et al., 2020	N/A
S2X35	Piccoli et al., 2020	N/A
Sotrovimab	GSK; Primary paper: Pinto et al., 2020	N/A
Bacterial and virus strains
SARS-CoV-2 Wu01 pseudovirus	Vanshylla et al., 2021	N/A
SARS-CoV-2 BA.1 pseudovirus	Gruell et al, 2022a	N/A
SARS-CoV-2 BA.1.1 pseudovirus	This paper	N/A
SARS-CoV-2 BA.2 pseudovirus	This paper	N/A
SARS-CoV-2 BA.2.12.1 pseudovirus	This paper	N/A
SARS-CoV-2 BA.4/5 pseudovirus	This paper	N/A
Biological samples
COVID-19 convalescent cohort serum samples	This paper; Vanshylla et al., 2021	N/A
Vaccinated cohort serum samples	This paper; Hillus et al., 2021	N/A
Chemicals, peptides, and recombinant proteins
ATP	Sigma-Aldrich	Cat#A2383; CAS: 34369-07-8
Branched polyethylenimine, 25 kDa	Sigma-Aldrich	Cat#408727; CAS: 9002-98-6
Coenzyme A sodium salt hydrate	Sigma-Aldrich	Cat#C3144; CAS: 55672-92-9
D-Luciferin, sodium salt	GoldBio	Cat#LUCNA-1G; CAS: 103404-75-7
Dulbecco’s Modified Eagle Medium (DMEM)	Thermo Fisher	Cat#11960044
Fetal bovine serum (FBS)	Sigma-Aldrich	Cat#F9665
FreeStyle™ 293 Expression Medium	Thermo Fisher	Cat#12338001
FuGENE® 6 Transfection Reagent	Promega	Cat#E2691
IGEPAL	Sigma-Aldrich	Cat#I8896; CAS: 9002-93-1
L-Glutamine	Thermo Fisher	Cat#25030024
MgCl2	Sigma-Aldrich	Cat#M8266; CAS: 7786-30-3
NEBuilder® HiFi DNA Assembly Cloning Kit	New England Biolabs	Cat#E5520S
Penicillin-Streptomycin	Thermo Fisher	Cat#15140122
Protein G Sepharose 4 Fast Flow	Cytiva	Cat#17061805
Q5® Site-Directed Mutagenesis Kit	New England Biolabs	Cat#E0554
Sodium pyruvate	Thermo Fisher	Cat#11360070
Experimental models: Cell lines
293T-ACE2 cells	Jesse Bloom Lab; Crawford et al., 2020; BEI Resources	Cat#NR-52511
293-6E cells	National Research Council of Canada	NRC file 11565
HEK293T cells	ATCC	Cat#CRL-11268
Recombinant DNA
Antibody heavy and light chain cloning fragments	This paper; Integrated DNA Technologies	N/A
Human antibody expression vectors (IgG1, Igκ, Igλ)	Tiller et al., 2008	N/A
pcDNA™3.1/V5-His TOPO™ TA Expression Kit	Thermo Fisher	Cat#K480001
pHAGE-CMV-Luc2-IRES-ZsGreen-W	Jesse Bloom Lab; Crawford et al., 2020	RRID: Addgene_164432
pHDM-Hgpm2	Jesse Bloom Lab; Crawford et al., 2020	RRID: Addgene_164441
pHDM-tat1b	Jesse Bloom Lab; Crawford et al., 2020	RRID: Addgene_164442
pRC-CMV-Rev1b	Jesse Bloom Lab; Crawford et al., 2020	RRID: Addgene_164443
SARS-CoV-2 spike gene fragments	This paper; Thermo Fisher	N/A
Software and algorithms
ChimeraX-1.3	Goddard et al., 2018; Pettersen et al., 2021	RRID: SCR_015872
Codon Usage Database	Nakamura et al., 2000	N/A
Codon Optimization Tool	Integrated DNA Technologies	N/A
EMBL-EBI search and sequence analysis tools API	Madeira et al., 2019	N/A
Geneious Prime	Biomatters	RRID: SCR_010519
IgBlast	National Library of Medicine; Ye et al., 2013	RRID: SCR_002873
MAFFT algorithm	Katoh et al., 2002	N/A
Matplotlib	http://matplotlib.sourceforge.net/	RRID: SCR_008624
NumPy	https://numpy.org	RRID: SCR_008633
Pandas	https://pandas.pydata.org	RRID: SCR_018214
Prism	GraphPad	RRID: SCR_002798
Python	http://www.python.org/	RRID: SCR_008394
SciPy	http://www.scipy.org/	RRID: SCR_008058
Sequence Manipulation Suite	Stothard,2000	N/A
Other
COVID-19 Data Repository by the Center for Systems Science and Engineering (CSSE) at Johns Hopkins University (weekly SARS-CoV-2 infections)	Dong et al.,2020	https://coronavirus.jhu.edu
CoV-AbDab (The Coronavirus Antibody Database)	Raybould et al.,2021	http://opig.stats.ox.ac.uk/webapps/covabdab/
GISAID (deposited SARS-CoV-2 sequences by variant)	Elbe and Buckland-Merrett, 2017; Khare et al., 2021; Shu and McCauley, 2017	https://www.gisaid.org; RRID: SCR_018279
IMGT Database	Lefranc,2011	https://www.imgt.org; RRID: SCR_012780
Our World in Data (aggregated weekly SARS-CoV-2 infections)	Global Change Data Lab	https://ourworldindata.org