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. 2022 Jun 23;13:914138. doi: 10.3389/fpls.2022.914138

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Copyright © 2022 Božunović, Milutinović, Aničić, Skorić, Matekalo, Živković, Dragićević, Filipović, Banjanac, Petrović and Mišić.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Maps of vectors used in this study, pRSETA for the expression in E.coli (A) and pJL-TRBO for overexpression in C. erythraea (B). CeBGlu, β-D-glucosidase from C. erythraea, was inserted as a XhoI-KpnI fragment into pRSETA expression vector (A), or as PacI-AvrII fragment into pJL-TRBO (B) to produce pRSETA:CeBGlu and pJL-TRBO:CeBGlu constructs, respectively. The T-DNA regions of binary plasmids are presented. Block arrow, CaMV duplicated 35S promoter and T7 promoter. Dark blue box, CaMV polyA signal sequence/terminator. Light blue box, Ribozyme. Bent arrows, Subgenomic promoters. ORFs are represented by colored boxes and their identities are labeled in boxes. Replicase, TMV 126K/183K ORF; MP, movement protein; RBS, ribosome binding site; Colored oval boxes present start codon (ATG) and polyhistidine-tag sequence (6xHIS).