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. 2022 Jun 23;13:914138. doi: 10.3389/fpls.2022.914138

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Copyright © 2022 Božunović, Milutinović, Aničić, Skorić, Matekalo, Živković, Dragićević, Filipović, Banjanac, Petrović and Mišić.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Agroinfiltration of leaves of diploid (A) and tetraploid (B) C. erythraea plants. Changes in the content of secoiridoid glucosides (SGs) swertiamarin (SWM), sweroside (SW) and gentiopicrin (GP) 5 days following agroinfiltration were recorded: CeBGlu1 and CeBGlu2 cloned into pJL-TRBO expression vector, were co-expressed either without or with silencing inhibitor p19 (pBIN:p19 construct). Results are expressed as means ± SE. Red asterisks denote significantly different values according to the t-test, p-values, *p < 0.05, **p < 0.01.