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Retaining double displacement reaction mechanism of GH1 β-glucosidase enzymes. Glu187 is the catalytic proton donor which activates the leaving group while Glu420 residue is the nucleophile. The reaction proceeds via a covalent glycosyl enzyme intermediate with an inverted bond which is hydrolyzed by nucleophilic attack of a water molecule, activated by the proton donor residue, resulting in second bond inversion and in overall anomeric retention.
