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. 2022 Jun 23;13:914971. doi: 10.3389/fmicb.2022.914971

Figure 1.

Figure 1

TRAF3IP3 interacts with EV71 3Cpro. (A) HEK293T cells were co-transfected with GFP-3C-C147S and HA-TRAF3IP3. Cells were harvested 48 h after transfection and co-immunoprecipitated using anti-HA antibodies, and protein interactions were detected using Western blotting. WCL, whole-cell lysate. (B) HEK293T cells were co-transfected with HA-TRAF3IP3 and Myc-3C-C147S. Anti-Myc antibodies were used for co-immunoprecipitation, and other operations were the same as in (A). (C) HA-TRAF3IP3 or Myc-3C-C147S were transfected into HeLa cells. 48 h later, the cells were fixed and protein subcellular localizations were observed using immunofluorescence. Scale bars = 10 μm.