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. 2022 Jun 23;13:914971. doi: 10.3389/fmicb.2022.914971

Figure 2.

Figure 2

TRAF3IP3 is the cleavage substrate for 3Cpro. (A) HA-TRAF3IP3 or GFP was co-transfected with 3Cpro or C147S into HEK293T cells. Cells were harvested 48 h later for Western blotting. (B) HEK293T was co-transfected with HA-TRAF3IP3 and a gradient of Myc-3C, with C147S as a non-cleavable control. The cleavage bands were detected using the antibody recognizing the C-terminus of TRAF3IP3. (C) EV71 at an MOI of 10 was used to infect into Jurkat cells for 0, 4, 8, 12, and 24 h, and the cleavage of endogenous TRAF3IP3 was detected using Western blotting. * and ** represent short and long exposures, respectively.