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. 2022 Jun 23;13:914971. doi: 10.3389/fmicb.2022.914971

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Copyright © 2022 Li, Yao, Chen, Zhang, Deng, Qiao and Tan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of the NLS and NES of TRAF3IP3. The subcellular localization of the protein was detected using immunofluorescence analysis. Cells were fixed 48 h after transfection. Scale bars represent 10 μm. (A) HeLa cells were transfected with HA-TRAF3IP3, HA-TRAF3IP3 1–87, or HA-TRAF3IP3 88–551. (B) Schematic representation of the pC3-EGFP-X-GST vector. HeLa cells were transfected with EGFP-X-GST vector, EGFP-SV40 NLS-GST, EGFP-TRAF3IP3 1–87-GST, or EGFP-TRAF3IP3 30–37-GST. (C) HeLa cells were transfected with HA-TRAF3IP3 or HA-TRAF3IP3 Δ30–37. (D) Schematic representation of the pC3-EGFP vector. HeLa cells were transfected with EGFP vector or EGFP-TRAF3IP3 407–416. (E) HeLa cells were transfected with HA-TRAF3IP3 or HA-TRAF3IP3 Δ407–416. (F) Schematic representation of the NLS and NES of TRAF3IP3.