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The NES of TRAF3IP3 contributes to the inhibition of EV71. (A) HA-TRAF3IP3 wild-type or deletion mutants were transfected into RD cells, followed by infection with EV71 at an MOI of 1 at 36 h post-transfection. Twelve hours later, the cells were collected for Western blotting. (B) HA-TRAF3IP3 wild-type or deletion mutants and Myc-3C-C147S were co-transfected into HeLa cells. Cells were fixed for immunofluorescence after 48 h. Scale bars represent 10 μm.
