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. 2022 Jun 23;13:886683. doi: 10.3389/fimmu.2022.886683

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Copyright © 2022 Grace, Backlund, Morgan, Kang, Singh, Huisman, Rappazzo, Moynihan, Maiorino, Dobson, Kyung, Gordon, Holec, Mbah, Garafola, Wu, Love, Wittrup, Irvine and Birnbaum

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sequencing of T cells from AIP-treated melanomas reveals significant expansion in the p15E-tetramer⁺ fraction. (A) Clone sizes in tetramer-positive and tetramer-negative populations from TILs isolated from AIP-treated mice as revealed by single-cell T cell sequencing. (B) Unsupervised gene expression analysis of CD8⁺ TILs reveals five distinct transcriptomic clusters. (C) Distribution of tetramer-positive and tetramer-negative cells amongst the 5 clusters. (D) Clusters 1 and 5 show enrichment for expanded clonal TCR sequences. (E) Clone sizes of isolated TILs mapped onto the clusters as determined in (B). (F) Expression of a selection of T cell effector and phenotype genes for each transcriptomic cluster. Scaled expression of common genes is indicated by color, and the fraction of cells in that cluster expressing the gene is indicated by relative dot size.