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. 2022 Jun 23;13:893803. doi: 10.3389/fimmu.2022.893803

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Copyright © 2022 van Unen, Ouboter, Li, Schreurs, Abdelaal, Kooy-Winkelaar, Beyrend, Höllt, Maljaars, Mearin, Mahfouz, Witte, Clemens, Abraham, Escher, Lelieveldt, Pascutti, van der Meulen – de Jong and Koning

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Imaging-mass cytometry reveals immune cell aggregates in the inflamed intestine. Representive images (A) structural markers (E-cadherin, Ki67, DNA, Collagen I, CD56, CD31, SMA) and CD45 expression are depicted for IBD biopsies from a control and three IBD patients, yellow scale bar: 100 μm. (B) Major immune lineage markers (CD4, CD11c) together with E-cadherin present in a region of a control and three IBD biopsies (dotted line in panel (A), yellow scale bar: 50 μm. (C) Co-expresion of CD3 and CD45RO with CD4, CD16 with CD66b, and HLA-DR with CD11c in the same region highlighted in panel (A) for a control and an IBD patient biopsy; scale bar: 50 μm.