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. 2022 Jun 23;13:868081. doi: 10.3389/fmicb.2022.868081

Table 1.

Dominant endophytes from plants growing across salinity gradients.

Plant species and location Soil conductivity* Endophyte morphotype** Salt stress category*** Endophyte identity****
Pickleweed (Salicornia pacifica), Picnic Cove, Shaw Island
Low Tide Zone 2,750 uS/cm A Extreme Phoma sp.
Mid Tide Zone 1,282 uS/cm A/B High Phoma sp./F. culmorum
High Tide Zone 701 uS/cm B Moderate F. culmorum
Dunegrass ( Leymus mollis ), Cedar Rocks, Shaw Island
Beach 436 μS/cm B Moderate F. culmorum
Slope 249 μS/cm C Low Fusarium redolens
Upper Meadow 84 μS/cm D Low Alternaria sp.
Dunegrass ( Leymus mollis ), Cowlitz Bay, Waldron Island
Beach 364 μS/cm B Moderate F. culmorum
Slope 32 μS/cm E Low Fusarium sp. 1
Upper 43 μS/cm F Low Fusarium sp. 2
Saltgrass ( Distichlis spicata ), Cowlitz Bay, Waldron Island
Low Tide Zone 23,70 μS/cm A Extreme Phoma sp.
Mid Tide Zone 1,801 μS/cm A/B High Phoma sp./F. culmorum
High Tide Zone 702 μS/cm B Moderate/High Fusarium culmorum
Spartina ( Spartina anglica ), Camano Island
Tidal Channel 4,000 μS/cm A Extreme Phoma sp.
Slope 3,628 μS/cm A/B Extreme Phoma sp./F. culmorum
Mud Flat 1,600 μS/cm B Moderate/High F. culmorum

Salinity measurements were taken using a conductivity meter from soils in a central point where plants grew in abundance (*) and 10–30 plants were collected from each population for endophyte analysis. Endophytes were first placed into morphotype groups after microscopic analysis (**). Deionized water equates to 1.0 μS/cm, rainwater average is 50–100 μS/cm, and seawater is approximately 50,000 μS/cm. Salt stress habitats were categorized as extreme at conductivity reads <2,500 μS/cm, high at 700–2,499 μS/cm, moderate at 200–699 μS/cm, and low at a range of 0–199 μS/cm (***). Isolates were then further identified using DNA sequence analysis (****).