Table 1.

Number and collection time of cells for single-cell RNA-seq analysis.

Stage	No. of embryos	No. of single cells	Collected hours*
Zygote	3	3	72 h
2-cell	2	4	84–90 h
4-cell	2	4	96–102 h
8-cell	2	4	114–120 h
Morula	2	4	150–156 h
Total	11	19

^*Collected N hours after estrus.