Skip to main content
. 2022 May 23;94(26):9316–9326. doi: 10.1021/acs.analchem.2c00754

Figure 1.

Figure 1

Comparison of dynamically harmonized MALDI FT-ICR MSI acquired on a solariX XR 9.4T at 4M with a manufacturer recommendation-based method (A) and a 6 laser shot-based method (B); see Section 3.2 for details. Reconstructed heat maps of the non-normalized total ion count of MS images (a). Portions of the TIC over time of the MSI acquisition and the computed mean intensities with standard deviation (b). Observable gaps on the TIC are values from pixels outside of the tissue section and were excluded to compute the standard deviation. The TIC presented in the top panel was obtained when 10 nmol·mm–2 α-HCCA matrix was deposited using 98% laser focus and from 5 nmol·mm–2 α-HCCA matrix with 80% laser focus (bottom panel). Multipixel mass spectra overlay of m/z 782.5674 shows a notable improvement in terms of mass R.P. and mass accuracy measurements during the MALDI images between the unstable (top panel) and stable (bottom panel) total ion count (c).