Figure 2.

Heat map of the non-normalized TIC of a mouse brain section analyzed by high-resolution MALDI FT-ICR MSI on a solariX XR 9.4T using nonoptimized MSI method (a). Extracted mass spectra from single pixels located in regions with significant differences in total ion current (b). Average mass spectrum (mean spectrum) of the whole MALDI image (c). Zoomed profile average spectrum focused on m/z 782.57 and 942.64 showing artifacts of split peaks and their complementary distributions due to inconsistent mass measurement accuracy during acquisition (d). Obtained localizations with a window selection encompassing both m/z peaks shown in the vicinity of m/z 782.57 and 942.64, respectively (e).