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. 2022 May 23;94(26):9316–9326. doi: 10.1021/acs.analchem.2c00754

Figure 4.

Figure 4

Centroided average MSI mass spectra of mouse brain (a) and zebrafish (b) using a MALDI FT-ICR (solariX XR 9.4T, fitted with ParaCell) instrument, zoomed in on m/z 788.62 and 798.54, respectively, showing peak width differences due to MMA obtained with (red) and without (green) TIC stabilization by optimization of the laser shot number (6 laser shots at 60 Hz). Reconstructed brain slices MS images without (c) and with (d) TIC normalization. Reconstructed zebrafish whole-body slices MS images with TIC normalization (e, TIC stabilization method (up) and no TIC stabilization (down), respectively). Section of the TIC over time to show the TIC stability of the presented MSI (f). Centroided average MSI mass spectrum of a mouse brain tissue section acquired on the scimaX 2XR using the 2ω detection mode with TIC stabilization (g). Zoom in to m/z 798.54 showing peak width difference due to MMA (h). Zoom in on m/z 798.54 in an extracted pixel spectrum showing the obtained R.P. FWHM and MMA (i). See text for details.