Table 3. Intensities and Ratio of Detected and Identified Lipids in a Mouse Brain Tissue Section Acquired with the MALDI-ToF MS (rapifleX) Instrument (External Calibration, Enhanced Cubic Regression) for 10 and 1000 Laser Shots with the Single Focus Option and Without Beamscan.
| 10 shots |
1000
shots |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| target m/z | identification | intensity (c.p.s) | mass accuracy (ppm) | ratio m/z 782.57 over m/z target | ratio m/z 798.54 over m/z target | Intensity (c.p.s) | Mass accuracy (ppm) | Ratio m/z 782.57 over m/z target | Ratio m/z 798.54 over m/z target |
| 782.567 | [PC 36:4 + H]+ | 6.8 × 103 | +9.71 | 0.79 | 1.8 × 104 | +7.1 | 0.81 | ||
| 798.541 | [PC 34:1 + K]+ | 5.3 × 103 | +11.2 | 1.27 | 1.5 × 104 | +10.0 | 1.23 | ||
| 806.567 | [PS 37:0 + K]+ | 4.0 × 103 | –12.3 | 1.68 | 1.33 | 9.7 × 103 | –11.1 | 1.90 | 1.54 |
| 844.546 | [PC 36:3 + Na]+ | 4.6 × 103 | +3.8 | 1.47 | 1.16 | 1.5 × 104 | 6.2 | 1.22 | 0.99 |