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. 2022 Jun 24;24(2):280. doi: 10.3892/ol.2022.13400

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Copyright: © Luo et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — PVT1 knockdown reduces 5-FU resistance in HCT116-5FU-resistant cells (A) RT-qPCR was used for the detection of knockdown efficiency of PVT1 siRNAs in HCT116-5FU-resistant cells, (B) CCK-8 assay was used to detect the impact of PVT1 knockdown on the 5-FU IC₅₀ values of HCT116-5FU-resistant cells. (C) CCK-8 assay was used to examine the effect of PVT1 knockdown on the proliferation of HCT116-5FU-resistant cells treated with or without 1 mM 5-FU. (D) Clone formation assay was used to evaluate the effects of PVT1 knockdown on stem like properties of HCT116-5FU-resistant cells treated with or without 1 mM 5-FU. (E) Flow cytometric analysis was used for the evaluation of PVT1 knockdown effect on cell cycle in HCT116-5FU-resistant cells treated with or without 1 mM 5-FU. *P<0.05. ns, not significant; PVT1, plasmacytoma variant translocation 1; 5-FU, 5-fluorouracil; RT-qPCR, reverse transcription-quantitative PCR.