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. 2022 Jun 24;24(2):280. doi: 10.3892/ol.2022.13400

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Copyright: © Luo et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — PVT1 adsorbs miR-486-5p for the upregulation of CDK4 expression. (A) Volcano map demonstrating differentially expressed miRNAs in colon cancer from the GEO database. (B) Dual luciferase experiments verified the interaction of PVT1 and miR-486-5p in 293T cells. (C) RNA pull-down experiment verified the interaction between PVT1 and miR-486-5p in HCT116-5FU-resistant cells with PVT1-WT and PVT1-MUT probe. (D) An AGO2-RIP experiment verified the binding of PVT1 to miR-486-5p in 5-HCT116-5FU-resistant cells transfected PVT1-WT and PVT1-MUT. (E) Reverse transcription-quantitative PCR was used for the evaluation of PVT1 effect on miR-486-5p. *P<0.05. ns, not significant; PVT1, plasmacytoma variant translocation 1; GEO, gene expression omnibus; 5-FU, 5-fluorouracil.