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. 2022 Jun 24;24(2):280. doi: 10.3892/ol.2022.13400

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Copyright: © Luo et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — PVT1 adsorbs miR-486-5p to upregulate CDK4 expression. (A) A Venn map was created using GEO data along with TargetScan, miRDB to screen for miR-486-5p target genes (B) A dual luciferase experiment verified the interaction between miR-486-5p and the CDK4 3′UTR in 293T cells. (C) Western blot analysis was used for the detection of CDK4 expression in parental and 5FU-resistant HCT116 cells. (D) Reverse transcription-quantitative PCR was used to detect PVT1, miR-486-5p and CDK4 expression in parental and 5FU-resistant HCT116 cells. (E) Western blot analysis was performed to estimate the CDK4 protein levels in 5FU-resistant HCT116 cells treated with or without 1 mM 5-FU. *P<0.05. PVT1, plasmacytoma variant translocation 1; GEO, gene expression omnibus; 5-FU, 5-fluorouracil; HAT1, histone acetyltransferase 1; TWF1, twinfilin actin binding protein 1.