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. 2022 Jun 17;7(26):22743–22753. doi: 10.1021/acsomega.2c02260

Figure 2.

Figure 2

DANCR expression and downstream effects of silencing. (A) The NCI-H1299 cell line expresses DANCR mRNA at a 1.5× greater level relative to the A549 cell line as determined with a qRT-PCR analysis (n = 3). (B) Treatment with RGD-PEG-ECO/siDANCR nanoparticles silence DANCR expression over 90% relative to control (n = 3). (C) DANCR silencing in the NCI-H1299 cell line persists over the course of 6 d, with strongest silencing observed at 2 d post-transfection (n = 3). (D) Markers of epithelial-to-mesenchymal transition (EMT) are reduced on the mRNA level following DANCR silencing with ECO/siDANCR nanoparticles as shown with qRT-PCR (n = 3). (E) On the protein level, the same markers as in (D) similarly show reduced expression after silencing DANCR (n = 3). Quantification of western blot images shown as a ratio of the band of interest normalized to the loading control, β-actin. Error bars denote standard error of measure, * p < 0.05, ** p < 0.01, and *** p < 0.005 using unpaired t-test.