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. 2022 Jun 17;7(26):22743–22753. doi: 10.1021/acsomega.2c02260

Figure 3.

Figure 3

ECO/siDANCR nanoparticles inhibit lung cancer cell migration and invasion in Transwell assays. Across both A549 (A) and NCI-H1299 (C) cell lines (n = 3), cell migration (without Matrigel coating) and invasion (with Matrigel coating) are reduced upon silencing DANCR expression. Counting the number of stained cells migrating or invading across the Transwell membrane quantifies the reduction in migration and invasion for A549 (B) and NCI-H1299 (D) cells. Wound-healing assays revealed slower migration after DANCR knockdown in A549 (E) and NCI-H1299 (G) (n = 3). Quantification of wound-healing assay images as distance migrated relative to the original boundary positions over the course of the experiment for both A549 (F) and NCI-H1299 (H). (I) 3D growth on Matrigel after 5 d revealed smaller spheroid size after DANCR knockdown (n = 3). (J) Staining of the spheroids in (I) with ZD2-Cy5.5 (red) and Hoechst 33342 (blue) and subsequent confocal imaging showed the relative expression of EDB protein in the extracellular matrix. (K) Ratio of Cy5.5 maximum intensity to Hoechst maximum intensity in staining from (J) was reduced with DANCR silencing. Error bars denote the standard error of measure; scale bars in all panels = 100 μm. “NC” represents negative control RGD-PEG-ECO/siNS, “siD” represents RGD-PEG-ECO/siDANCR. * p < 0.05, ** p < 0.01, and *** p < 0.005 using unpaired t-test.