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. 2022 Jun 17;7(26):22743–22753. doi: 10.1021/acsomega.2c02260

Figure 5.

Figure 5

DANCR silencing with RGD-PEG-ECO/siDANCR is sustained over time. (A) RGD-PEG-ECO/siDANCR nanoparticles showed a functional effect in reducing cell viability after transfection relative to RGD-PEG-ECO/siNS nanoparticles or untreated control groups in both NCI-H1299 and A549 cells (n = 3). Viability was expressed relative to untransfected cells. (B) A single 48 h transfection with RGD-PEG-ECO/siDANCR was sufficient to reduce DANCR expression in A549 and NCI-H1299 cells for 7 d relative to a single 48 h transfection with RGD-PEG-ECO/siNS (n = 3). (C) Cell migration and invasion (n = 3) assessed 7 d following the initial transfection performed in (B) showed a greater reduction in NCI-H1299 cells than in A549 cells, where DANCR expression recovered to 20% and 50%, respectively. (D) Quantification of the number of cells migrated or invaded in A549 DR or NCI-H1299 DR cells. (E) In drug-resistant NSCLC cells, a single transfection of RGD-PEG-ECO/siDANCR showed a reduction in DANCR expression lasting for 7 d and fully recovering to pretransfection level at 10 d (n = 3). Error bars denote standard error of measure, scale bars = 100 μm. * p < 0.05, ** p < 0.01, and *** p < 0.005 using unpaired t-test.