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. 2022 Jul 6;10(7):e004616. doi: 10.1136/jitc-2022-004616

Figure 2.

Figure 2

Inhibition of SCD1 enhances the infiltration of CD8+ T cells and DCs into tumors via production of CCL4. C57BL/6 mice bearing MC38 or MCA205 tumors and Balb/c mice bearing CT26 or 4T1 tumors were treated with a SCD inhibitor (SCDinh) or with vehicle only (mock). Tumors were excised on day 20. (A) Analysis of tumor-infiltrating CD8+ T cells by qPCR, flow cytometry, immunostaining and image analysis (n=5). (B) Percentages of 4-1BB+, PD-1+, TIGIT+ and Lag3+ CD8+ T cells in tumors analyzed by flow cytometry in C57BL/6-MC38 model (n=5). (C) Absolute numbers of CD45+ CD11c+ cells analyzed by flow cytometry (n=5). (D, E) Ccl4 and Atf3 gene expression evaluated by real-time RT-PCR (n=5). *P<0.05, **P<0.01. N.D., not determined. Data are expressed as means ± SD.