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. Author manuscript; available in PMC: 2022 Nov 17.
Published in final edited form as: Biochemistry. 2022 May 6;61(10):843–855. doi: 10.1021/acs.biochem.1c00740

Table 5.

Rate Constants Governing Nucleotide Binding to SULT2B1b

Nucleotide (−) Quc (+) Quc
kon (μM−1s−1) koff (s−1) kon (μM−1s−1) koff (s−1)
PAPS 8.0 (0.3)1 3.0 (0.2) 8.2 (0.5) 0.22 (0.02)
2Kd PAPS 0.38 (0.02) 0.027 (0.003)
1

Values in parentheses indicate standard error.

2

Kd = koff/kon.