KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit-anti-RFP | Rockland | Cat# 600-401-379; RRID:AB_2209751 |
| Goat-anti-HB-EGF (anti-DTR) | R&D Systems | Cat# AF-259-NA, RRID:AB_354429 |
| Rabbit-anti-Fos | Synaptic Systems | Cat# 226 003, RRID:AB_2231974 |
| Chick-anti-GFP | Aves Labs | Cat# GFP-1020, RRID:AB_10000240 |
| Bacterial and virus strains | ||
| AAV9-pCAG-Flex-EGFP | Oh et al., 2014 | Addgene, Cat #51502-AAV9 |
| AAV9-Ef1a-DIO-hChR2(H134R)-mCherry | Gift from Karl Deisseroth | Addgene, Cat #20297-AAV9 |
| pAAV-hSyn-DIO-rM3D (Gαs)-mCherry | Gift from Bryan Roth | Addgene, Cat #50458 |
| AAV8.2-hEF1a-DIO-synaptophysin-mCherry | MGH Viral Core | Cat #AAV-RN1 |
| Green Up cADDis cAMP sensor | Montana Molecular | Cat #U0200G |
| Chemicals, peptides, and recombinant proteins | ||
| [D-Ala2]-GIP | Tocris | Cat #6699 |
| Clozapine N-oxide dihydrochloride | Tocris | Cat #6329 |
| Bicuculline methiodide | Tocris | Cat #2503 |
| Cyanquixaline | Tocris | Cat #1045 |
| Forskolin | Tocris | Cat #1099 |
| Diphtheria Toxin from Corynebacterium diphtheriae | Sigma-Aldrich | Cat #D0564 |
| D-AP5 | Tocris | Cat #0106 |
| GDF15 | R&D systems | Cat #957-GD-025/CF |
| Tetrodotoxin | Tocris | Cat #1078 |
| Gipr B3 probe | Molecular Instruments | Lot #PRL060 |
| FluoroGold | Santa Cruz Biotechnology | Cat #sc-358883 |
| Critical commercial assays | ||
| Papain dissociation system | Worthington Biochemical | Cat # LK003150 |
| Deposited data | ||
| Raw images | This study | Mendeley data https://doi.org/10.17632/h3fztb3ynh.1 |
| Experimental models: Organisms/strains | ||
| Glplr-ires-Cre | Chang et al., 2015; Williams et al.,2016 | Deposited in Jackson laboratory (Cat#029282) |
| Calcr-ires-Cre | Pan et al., 2018 | N/A |
| Gipr-Cre | Adriaenssens et al., 2019 | N/A |
| Wild-type C57BL/6J | Jackson Laboratory | Cat #000664 |
| Rosa26-LSL-L10GFP | Jackson Laboratory | Cat #024750 |
| Agtr1a-t2a-Cre | Jackson Laboratory | Cat #031487 |
| Rosa26-LSL-tdTomato | Jackson Laboratory | Cat #007908 |
| Gad2-ires-Cre | Jackson Laboratory | Cat #010802 |
| Rosa26-LSL-DTR | Jackson Laboratory | Cat #007900 |
| Slc6a2-p2a-Cre | Zhang et al., 2021 | N/A |
| Gfral-p2a-Cre | Zhang et al., 2021 | N/A |
| Software and algorithms | ||
| MATLAB R2019a | MathWorks | RRID: SCR_001622 https://www.mathworks.com/products/matlab.html |
| PRISM 9 | GraphPad | RRID: SCR_002798 https://www.graphpad.com/scientific-software/prism/ |
| FIJI | Schindelin et al., 2012 | RRID:SCR_002285 https://fiji.sc |
| pClamp 10.2 | Axon Instruments | RRID: SCR_011323 https://www.moleculardevices.com/products/software/pclamp.html |
| BioRender | BioRender.com | RRID: SCR_018361 https://biorender.com |