Table 1. Expected Poly(A) Length in GFP DNA Templates Compared to the Observed Weighted Average Poly(A) Length Using Our Methoda.
| targeted Poly(A) length (nt) | weighted average Poly(A) length by mass spectrometry (nt) | expected size on tapestation (bp) | measured size on tapestation (bp) |
|---|---|---|---|
| 0 | 0 | 187 | 192 |
| 50 | 55.2 | 214 | 218 |
| 80 | 85.2 | 244 | 244 |
| 100 | 104.3 | 264 | 258 |
| 120 | 120.7 | 284 | 278 |
| 150 | 140.2 | 314 | 301 |
a
The DNA fragments were cleaved with a restriction enzyme and the expected fragment size for the poly(A) containing species is listed as well as the calculated size by the TapeStation. The size of the restriction fragment without a poly(A) tail is expected to be 187 nt.