Figure 5.

LLS, TEM, and ELISA endpoint measurements of P to S mutations induced by P100. (A) Endpoint total amount of induced aggregation of each P to S mutation at 2 μM induced with ∼1.4 μM P100 quantified using LLS (n = 3 ± s.d). TEM micrographs selected at random were quantified to measure the (B) total filament mass of each micrograph ± s.d, (C) average number of filaments (>25 nm) per micrograph ± s.d., and (D) average induced filament length (>25 nm) ± s.d. Immunoreactivity as measured by sandwich ELISA using capture antibodies TOC1 (E), TNT1 (F), and T22 (G). In figures (E–G), the y-axis represents the % light absorbed value (converted from A₄₅₀ reading). Error bars represent ± s.d. of three independent experiments. All results were compared to average measurements of 2 μM WT tau induced with ∼1.4 μM P100 ± 95% CI (gray shaded box) by an unpaired t-test (p < 0.05*, p < 0.01**, and p < 0.001***).