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. 2022 Jul 7;17:344. doi: 10.1186/s13018-022-03207-0

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Circ-FOXM1 targeted miR-320a and miR-320b to modulate FOXM1. a The Venn diagram of miRNAs binding to FOXM1 from four databases (PITA, miRanda, TargetScan and miRmap) included in ENCORI website. b Luciferase reporter assay was utilized to screen out the miRNA targeting FOXM1. c, d The mRNA and protein levels of FOXM1 were detected through qRT-PCR and western blot when miR-320a and miR-320b were overexpressed in OS cells. e ENCORI predicted the binding sites between miR-320a or miR-320b and FOXM1. f Luciferase reporter assay was applied for proving the binding site of miR-320a or miR-320b and FOXM1. g ENCORI predicted the binding sites between miR-320a or miR-320b and circ-FOXM1. h Luciferase reporter assay was applied for proving the binding site of miR-320a or miR-320b and circ-FOXM1. i RIP assay tested the enrichment of miR-320a, miR-320b, circ-FOXM1 and FOXM1 in Ago2-RISCs. **P < 0.01