Figure 4.

C3G enhanced promoter activity of CRE through the β₂AR signaling pathway. HEK293 cells were transiently co-transfected with pGL4.29 and pcDNA3.1-β₂AR then seeded into a 96-well plate. Transfected cells were treated with vehicle (0.1% DMSO, shown as −), isoproterenol (10 μM, shown as Iso), or C3G at the indicated concentrations for 24 h treatment. Luminescence value corresponding to CRE promoter activity was measured using cell lysates. Statistical analysis was conducted using one-way ANOVA (Tukey’s test). Significance differences (****p < 0.0001, **p < 0.01, *p < 0.05) versus vehicle treatment group at cells co-transfected with pGL4.29 and pcDNA3.1-β₂AR. Significant differences (####p < 0.0001, ##p < 0.01) versus vehicle treatment group at cells transfected with pGL4.29. n = 5.