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. 2022 Jun 22;7(26):22889–22895. doi: 10.1021/acsomega.2c02659

Figure 6.

Figure 6

Comparison study with aglycon. (A) NHDF cells were treated with vehicle (0.1% DMSO, shown as −), PDGF-BB (25 ng/mL, shown as P), C3G, or cyanidin at the indicated concentrations for 120 h. The amount of HGF secreted into the culture medium was measured by ELISA assay. Statistical analysis was conducted using Student’s t-test. Significance difference (**p < 0.01, *p < 0.05) versus vehicle treatment group. n = 5. (B) HEK293 cells were transiently co-transfected with pGL4.29 and pcDNA3.1-β2AR and then seeded into a 96-well plate. Transfected cells were treated with vehicle (0.1% DMSO, shown as −), isoproterenol (10 μM, shown as Iso), C3G, or cyanidin at the indicated concentrations for 24 h treatment. Luminescence was measured using cell lysates. Statistical analysis was conducted using one-way ANOVA (Tukey’s test). Significant difference (****p < 0.0001, **p < 0.01) versus vehicle treatment group. n = 5.