TABLE 1.
Transformation efficiencies in Propionibacterium strains with DNA isolated from E. coli or P. freudenreichii ATCC 6207a
| Recipient strain | Transformation efficiency (no. of transformants/μg of DNA)
|
|
|---|---|---|
| DNA isolated from E. coli | DNA isolated from P. freudenreichii | |
| P. freudenreichii strains | ||
| ATCC 6207 | 10–30 | ≥108 |
| VTB1 | 10–30 | ≥108 |
| LMG 16545 | 10–30 | ≥108 |
| P. acidipropionici strains | ||
| DSM 13572 | 0 | 0 |
| DSM 20727 | 0 | 0 |
| P. jensenii DSM 20535 | 0 | 0 |
| P. thoenii DSM 20276 | 0 | 0 |
a
Electroporation was performed at 20 kV/cm, 200 Ω, and 25 μF. The vectors used for transformation were pBRESP36A, pBRESP36B1, and pBRESP36B2.