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. 2022 Jan 13;2:828697. doi: 10.3389/fragi.2021.828697

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Copyright © 2022 Frasca, Romero, Garcia, Diaz and Blomberg.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of aging and obesity on lipid accumulation by B cells. PBMC were isolated from Y_L, E_L, Y_O and E_O individuals and stained first with the Deep Red Neutral Lipid Stain LipidTOX and then with anti-CD45, anti-CD19, and the Live/Dead detection kit. (A) MFI profiles of one representative donor/group. MFI values for negative controls (without the addition of LipidTOX are, respectively: 915 (Y_L), 1214 (Y_O), 837 (E_L), 1025 (E_O). (B) MFI data from all donors. Mean comparisons between groups were performed by two-way ANOVA: **p < 0.01, ****p < 0.0001.