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. 2022 Jan 13;2:828697. doi: 10.3389/fragi.2021.828697

FIGURE 4.

FIGURE 4

Palmitate in vitro increases mRNA expression of T-bet, the transcription factor for autoimmune antibody production, in B cells from both YL and EL individuals. Total B cells (A) and naïve B cells (B), isolated from the same YL and EL individuals in Figure 3, were stimulated with CpG or CpG/anti-Ig respectively, alone or in the presence of palmitate, for 1 day. Then the mRNA was extracted and qPCR reactions run to evaluate the expression of tbx21. Results show qPCR values (2−ΔCt) of tbx21, normalized to GAPDH. Mean comparisons between groups were performed by two-way ANOVA: ***p < 0.001, ****p < 0.0001.